Testicular Volume; Indicator for Male Fertility in Albino Rats

.. Objective: To determinate that male fertility influenced by testicular volume in albino rats. Study Design: Experimental. Place and Duration of study: Institute of Basic Medical Sciences, Dow University of Health Sciences, Karachi, 10 months (November 2009 to August 2010). Methodology: Sixty four adult albino rats were obtained from animal house Jinnah Postgraduate Medical Centre for the study and divided into 2 groups. Group A received injection normal saline 1 cc intraperitoneally (IP) daily for 8 weeks. Group B received lead chloride in a dose of 10 mg/kg body weight IP daily. On the day of completion of treatment the animals were sacrificed testes along with epididymis removed and place in Petri dish. The length, breath and width of testes were measured with help of vernier caliper. The spermatozoa were obtained from cauda epididymis. Results: The mean ± SEM of volume testes in group A and B after eight week of treatment were 0.77142 ± 0.04778 cm3 & 0.11768 ± 0.01673 cm3 respectively. The volume of testes of group B was significantly decreased as compare to group B (P = 0.000). Mean ±. The mean ± SEM number of sperm cells million / ml in groups A and B after eight week of treatment was 7.65 ± 186706.553 & 1.84 ± 132792.770 respectively. Number of sperms in group B were significantly decreased as compared to group A (P = 0.000). Conclusion: There was relationship between volume of testes and male fertility. Key word: Testicular Volume, Male Fertility, Lead Chloride, Sperms, Albino Rats, Cauda Epididymis 1. Assistant Professor Anatomy Al-Tibri Medical College, Isra University, Karachi Campus 2. Assistant Professor Anatomy Al-Tibri Medical College, Isra University, Karachi Campus 3. Professor, Anatomy Al-Tibri Medical College, Isra University, Karachi Campus 4. Lecturer Community Medicine Al-Tibri Medical College, Isra University, Karachi Campus Correspondence Address: Dr. Muhammad Rafique Plot No, S 44-A, Jami Staff Lane 2, DHA Phase II Extension [email protected] Article received on: 01/06/2013 Accepted for publication: 15/07/2015 Received after proof reading: 12/10/2015 Article Citation: Khan H, Mazhar Ul Haq, Rafique M, Jiskani AR. Testicular volume; indicator for male fertility in albino rats. Professional Med J 2015;22(10):1356-1361. DOI: 10.17957/TPMJ/15.2283 INTRODUCTION Assessment of testicular volume provides useful information about the spermatogenesis.1 The testicular volume reflects the spermatogenesis, as about 70 to 80 % of testicular mass generated by the seminiferous tubules. Testicular volume is indicating the male fertility and helpful for semen profiles in infertile men, and it has been useful in estimating spermatogenesis. The different methods to measure the testicular volume are vernier calipers, orchidometers and ultrasonography (US). Orchidometry is a conventional method and has been used for about 40 years; currently ultrasonography is the most popular and accurate technique to calculate the testicular volumes and can be used for actual testicular volume.2 Sexual maturation and differentiation is a continuous process starting early at the time of fertilization and during the 7th & 12th after fertilization. At time of puberty the process of sexual maturation is indicated by rapid and accelerated somatic development and increased secretion of male sex hormones. At time of puberty the earliest secondary sex characteristic which in male observed in male are increase in the size of testis, scrotum, and later an increase in the size of penis.3 There is evidence that sperm competition might have been important in the evolutionary lineage leading to humans. In primates, testis size is related to the mating system, the species having single male partner (monogamous species) they have smaller testes as compare to their body size as compare to species having multiple male partners (promiscuous).4 Those species have relatively larger testes, the motility of sperm motility is more5, and this leads to rapid evolution of genes which are involved in productions of sperm and seminal fluid.6 The size of testes in human is modThe Professional Medical Journal www.theprofesional.com DOI: 10.17957/TPMJ/15.2283